Archivo: Protease mechanisms summary

Descripción: A comparison of the two mechanisms used for proteolysis. enzyme is shown in black, substrate protein in red and water in blue.The top panel shows 1-step hydrolysis where the enzyme uses an acid to polarise water which then hydrolyses the substrate. The bottom panel shows 2-step hydrolysis where a residue within the enzyme is activated to act as a nucleophile (Nu) and attack the substrate. This forms an intermediate where the enzyme is covalently linked to the N-terminal half of the substrate. In a second step, water is activated to hydrolyse this intermediate and complete catalysis. Other enzyme residues (not shown) donate and accept hydrogens and electrostatically stabilise charge build-up along the reaction mechanism.
Título: Protease mechanisms summary
Créditos: Trabajo propio
Autor(a): Thomas Shafee
Términos de Uso: Creative Commons Attribution 4.0
Licencia: CC BY 4.0
Enlace de Licencia: https://creativecommons.org/licenses/by/4.0
¿Se exige la atribución?: Sí